| 质粒名称: | FUW mCherry-GFP-LC3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110060 |
| 存储实验室: | Anne Brunet |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry-GFP-LC3 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | TOPO克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CGCGGATCCGGTCGCCACCATGGTGAGCAAGGGCGAG |
| 3' 测序引物 | CGCGGCGCGCCGCTGGGTCTAGATGCATGC |
|
文献引用方法 |
|
| 材料和方法部分: | FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060 ; http://n2t.net/addgene:110060 ; RRID:Addgene_110060) |
| 参考文献部分: | Lysosome activation clears aggregates and enhances quiescent neural stem cell activation during aging. Leeman DS, Hebestreit K, Ruetz T, Webb AE, McKay A, Pollina EA, Dulken BW, Zhao X, Yeo RW, Ho TT, Mahmoudi S, Devarajan K, Passegue E, Rando TA, Frydman J, Brunet A. Science. 2018 Mar 16;359(6381):1277-1283. doi: 10.1126/science.aag3048. Epub 2018 Mar 15. 10.1126/science.aag3048 PubMed 29590078 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUW mCherry-GFP-LC3 11138 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION To visualize free autophagosomes (GFP and mCherry fluorescence) and
autophagosomes that have fused with the lysosome (autolyosomes;
mCherry fluorescence only, due to acid sensitivity of GFP).
ACCESSION .
VERSION .
KEYWORDS FUW mCherry-GFP-LC3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11138)
AUTHORS Leeman DS, Hebestreit K, Ruetz T, Webb AE, McKay A, Pollina EA,
Dulken BW, Zhao X, Yeo RW, Ho TT, Mahmoudi S, Devarajan K, Passegue
E, Rando TA, Frydman J, Brunet A
TITLE Lysosome activation clears aggregates and enhances quiescent neural
stem cell activation during aging.
JOURNAL Science. 2018 Mar 16;359(6381):1277-1283. doi:
10.1126/science.aag3048. Epub 2018 Mar 15.
PUBMED 29590078
REFERENCE 2 (bases 1 to 11138)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11138
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 7..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 388..586
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 604..784
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 831..956
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1449..1682
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2209..2326
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2391..3602
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3439..3458
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
regulatory 3641..3650
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3647..4354
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3792..3810)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4060..4079
/label=mCherry-F
/note="mCherry, forward primer"
protein_bind 4382..4406
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
regulatory 4415..4424
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4421..5137
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4466..4487)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4727..4746)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5074..5095
/label=EGFP-C
/note="EGFP, forward primer"
CDS 5165..5539
/codon_start=1
/gene="MAP1LC3B"
/product="human LC3 protein"
/label=LC3B
/note="Atg8 family member found on autophagosomes"
/translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
EDGFLYMVYASQETFGMKLSV"
misc_feature 5599..6187
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5652..5672)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6070..6081)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6190..6206)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6191..6207)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6712..6892
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6918..6935)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6924..7148
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7194..7622
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7281..7300)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7491..7512
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7631..7651)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7636..7965
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7816..7951
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7878..7897
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8013..8060
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8079..8453
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8583..8704
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8620..8639)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8674..8693
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8753..8769)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8753..8769)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8766..8788)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8777..8793
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8801..8831)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8846..8867
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8984..9001)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9155..9743)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9235..9254)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9914..10774)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10537..10556
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10775..10879)
/gene="bla"
/label=AmpR promoter
primer_bind complement(10954..10973)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
2341 ggacagcaga gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg
2401 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
2461 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
2521 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
2581 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
2641 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
2701 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
2761 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
2821 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
2881 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
2941 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
3001 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
3061 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
3121 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
3181 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
3241 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
3301 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
3361 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
3421 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
3481 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
3541 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
3601 acgaagcttg ggctgcaggt cgactctaga ggatccggtc gccaccatgg tgagcaaggg
3661 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
3721 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
3781 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
3841 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
3901 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
3961 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
4021 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
4081 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
4141 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
4201 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
4261 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
4321 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatccg ctggcccagg
4381 aacaagtttg tacaaaaaag caggctttcc ggtcgccacc atggtgagca agggcgagga
4441 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
4501 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
4561 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
4621 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
4681 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
4741 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
4801 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
4861 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
4921 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
4981 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
5041 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
5101 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ccggtaccga
5161 attcatgccg tcggagaaga ccttcaagca gcgccgcacc ttcgaacaaa gagtagaaga
5221 tgtccgactt attcgagagc agcatccaac caaaatcccg gtgataatag aacgatacaa
5281 gggtgagaag cagcttcctg ttctggataa aacaaagttc cttgtacctg accatgtcaa
5341 catgagtgag ctcatcaaga taattagaag gcgcttacag ctcaatgcta atcaggcctt
5401 cttcctgttg gtgaacggac acagcatggt cagcgtctcc acaccaatct cagaggtgta
5461 tgagagtgag aaagatgaag atggattcct gtacatggtc tatgcctccc aggagacgtt
5521 cgggatgaaa ttgtcagtgt aagtcgagca tgcatctaga cccagcggcg cgccgaattc
5581 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
5641 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
5701 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
5761 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
5821 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
5881 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
5941 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
6001 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
6061 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
6121 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
6181 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
6241 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
6301 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
6361 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
6421 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
6481 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
6541 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
6601 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
6661 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
6721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
6781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
6841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
6901 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
6961 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
7021 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
7081 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
7141 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
7201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
7261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
7321 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
7381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
7441 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
7501 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
7561 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
7621 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
7681 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
7741 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
7801 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
7861 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
7921 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
7981 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
8041 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
8101 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
8161 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
8221 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
8281 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
8341 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
8401 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
8461 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
8521 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
8581 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
8641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
8701 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
8761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
8821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
8881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
8941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
9001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
9061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
9121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
9181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
9241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
9301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
9361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
9421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
9481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
9541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
9601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
9661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
9721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
9781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
9841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
9901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
9961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
10021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
10081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
10141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
10201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
10261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
10321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
10381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
10441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
10501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
10561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
10621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
10681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
10741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
10801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
10861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
10921 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
10981 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
11041 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
11101 gcgttttgcg ctgcttcgcg atgtacgggc cagatata
//
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